Molecules with free DNA ends, but not circular molecules, play an important role in rAAV vector genome concatemerization Key Words adenoassociated virus, gene therapy, mouse, liver, inverted terminal repeat, recombination, integration, doublestranded DNA, naked DNA, Factor IX INTRODUCTION Adenoassociated virus (AAV) is a nonpathogenic replicaPapillomaviridae constitutes a diverse group of viruses with a circular doublestranded DNA (dsDNA) genome of approximately 8000 bp Papillomaviruses have two sets of genes expressed at different times during the replication cycle of the virus, ie the early genes (E17) and the late genes (L12) The early genes code for nonstructuralReverse transcriptase copies mRNA to make viral DNA RNA, single stranded, strand
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Do viruses have double stranded dna
Do viruses have double stranded dna-A) a linear DNA molecule containing 10,000 basepairs Both can be doublestranded, circular DNA b) Both can be singlestranded, linear RNA c) Both can be compacted into inverted repeats d) Both can be compactedCircular single stranded RNA double stranded RNA single stranded DNA all of the above are found in viruses How do viruses transport substances across their membrane active transport facillitated diffusion endocytosis pinnocytosis group translocation simple diffusion all of the above none of the above Viruses can be taken apart (separated) into
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25_____ A new virus, virus X, is isolated and studied Which molecule is most likely to be the genetic material of virus X?DNA, double stranded, circular (with singlestranded region), reverse transcriptase (RNAdependent DNA polymerase) Hepadnaviridae (enveloped) Cellular enzyme transcribes viral DNA in nucleus;Dec 08, 16 · Viruses strongly influence microbial population dynamics and ecosystem functions However, our ability to quantitatively evaluate those viral impacts is limited to the few cultivated viruses and doublestranded DNA (dsDNA) viral genomes captured in quantitative viral metagenomes (viromes)
Aug 10, 11 · The results reported here establish the novel finding that the AAV ssDNA is formed in yeast from a double stranded circular plasmid containing the ITRs Moreover, the plasmid is maintained through the generation as a double stranded circle in the absence of the 2 micron replication origin or ARS sequenceC SingleStrand DNA Viruses (eg, parvoviruses) Figure 11, p 110 from Fields Virology 1 Complementary DNA strand synthesized in nucleus 2 Transcription of the genome ensues D Partially Double Stranded DNA Viruses (hepadnaviruses) Figure 12, p 110 from Fields VirologyTo the Editor Circular replication initiation protein (Rep)–encoding singlestranded DNA (ssDNA) (CRESSDNA) genomes are found in diverse group II virus families, which all possess a conserved Repencoding gene and a nonenveloped icosahedral capsid, except geminiviruses, which have twinned particles ()Gemycircularvirus (GcV) were initially discovered in fungi, but a growing
Nudiviruses (NVs) are a highly diverse group of large, circular dsDNA viruses pathogenic for invertebrates They have rodshaped and enveloped nucleocapsids, replicate in the nucleus of infected host cells, and possess interesting biological and molecular properties The unassigned viral genus Nudivirus has been proposed for classification of nudivirusesCircular singlestranded DNA viruses infect archaea, bacteria, and eukaryotic organisms The relatively recent emergence of singlestranded DNA viruses, such as chicken anemia virus (CAV) and porcine circovirus 2 (PCV2), as serious pathogens of eukaryotes is due more to growing awareness than to the appearance of new pathogens or alteration of existing pathogensNov 26, 13 · Diatoms are among the most abundant organisms in nature;
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Virus Virus Annotated classification DNA viruses Family Poxviridae Large viruses of complex structure with dimensions of 400 × 250 nm, the genome of which is linear doublestranded DNA Virions contain at least 40 proteins and lipids, as well as internal structures called lateral bodies The 2 subfamilies are called Chordopoxvirinae, which infect vertebrates and are closely relatedDouble stranded DNA ‐convert gapped, genome to covalently closed, ds circular DNA This repair process must precede mRNA synthesis The unusual gapped DNA genome is the productof a curious replication Single stranded (ssDNA) genomes TT virus (ubiquitous human virus) B19 parvovirus (fifth disease) MID 30 RNA genomesProtocol to community virus DNA from three freshwater and three marine samples revealed that ssDNA viruses as a whole represent only a minor fraction (
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May 18, 04 · The sequence of the circular doublestranded DNA viral genome shows that it shares little similarity to other known genes in viruses or other organisms By comparing the tertiary and quaternary structures of the coat protein of this virus with those of a bacterial and an animal virus, we find conformational relationships among all threeOther papovaviruses include the papillomaviruses (family Papillomaviridae), which are also small polygonal viruses containing circular doublestranded DNA The papillomaviruses are associated with usually benign (nonthreatening) but widespread tumours, called papillomas or polyps, occurring in human skin and the genital tractRoux et al, 15) and circular contigs from 4–8 kb for Microviridae viruses or 1–5 kb for circular replicationassociated protein (Rep)encoding ssDNA (CRESS DNA) viruses
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Group I viruses contain doublestranded DNA (dsDNA) as their genome Their mRNA is produced by transcription in much the same way as with cellular DNA, using the enzymes of the host cell Group II viruses have singlestranded DNA (ssDNA) as their genomeJun 25, 17 · Herpesviridae is a large family of DNA viruses that cause diseases in animals, including humans The structure of herpes viruses consists of a relatively large doublestranded, linear DNA genome encased within an icosahedral protein cage called the capsid, which is wrapped in a lipid bilayer called the envelopeThe circular nature of plasmids and the circularization of some viral genomes on infection make this possible Rolling circle replication begins with the enzymatic nicking of one strand of the doublestranded circular molecule at the doublestranded origin (dso) site In bacteria, DNA polymerase III binds to the 3'OH group of the nicked
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Jul 31, 19 · While all cellular life relies on doublestranded DNA inheritance, viruses can be single or doublestranded and make use of either DNA or RNA asThese results suggest that TTV replicates in the liver via a circular doublestranded DNA TT virus(TTV) is an unenveloped, singlestranded, and circularDNA virus (12, 13, 22)Mar 21, 19 · Viral operational taxonomic units (vOTUs) were defined as nonredundant (ie, postclustering) viral contigs >10 kb for dsDNA viruses (from VirSorter categories 1 or 2;
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Circular DNA synonyms, Circular DNA pronunciation, Circular DNA translation, English dictionary definition of Circular DNA DNA A adenine T thymine C cytosine G guanine n A nucleic acid that carries the genetic information in cells and some viruses, consisting of two longJan 17, 19 · Smacoviridae is a family of small (~25 Kb) CRESSDNA (Circular Rep Encoding SingleStranded (ss) DNA) viruses These viruses have been found in faeces, were thought to infect eukaryotes and areFeb 13, 08 · The GpSGHV genome is a doublestranded circular DNA molecule of 190,032 bp containing 160 nonoverlapping open reading frames (ORFs), which are distributed equally on both strands with a gene density of one per 12 kb It has a high AT content of 72%
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Jul 31, 19 · While all cellular life relies on doublestranded DNA inheritance, viruses can be single or doublestranded and make use of either DNA or RNA as their genetic materialDoubleStranded DNA Viruses Plant doublestranded DNA viruses have circular genomes of about 72–80 kb with one discontinuity in one strand and one or more in the other strand;Human papilloma virus is a nonenveloped, double stranded, circular DNA virus Parvovirus B19 David J Hall B19, database# 1S58 Parvovirus B19 virus is part of the parvovirus family and causes a childhood rash called fifth disease or erythema infectiosum that is commonly called slapped cheek syndrome B19 is a nonenveloped virus whose
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Jun 25, 17 · Double stranded DNA viruses only infect lower species of plants, such as algae These viruses are huge dsDNA viruses with genomes ranging from 160 to 560 kb with up to 600 proteinencoding genes, making them distinctly different from viruses infecting higher plantsClassification of Viruses Viruses can store their genetic information in six different types of nucleic acid which are named based on how that nucleic acid eventually becomes transcribed to the viral mRNA (see Fig 1) capable of binding to host cell ribosomes and being translated into viral proteins In the diagrams below, () and () represent complementary strands of nucleic acidThe type of genetic material (DNA or RNA) and its structure (single or doublestranded, linear or circular, and segmented or nonsegmented) are used to classify the virus core structures Adenovirus (left) is depicted with a doublestranded DNA genome enclosed in an icosahedral capsid that is 90–100 nm across
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These small, doublestranded DNA viruses have many unique properties For example, the DNA is the only covalently closed circular DNA Morphology The virion is spherically shaped, naked and contains icosohedral symmetry (T = 7) The are the only icosohedrally symmetric virion which contains skew symmetryGroup I viruses contain doublestranded DNA (dsDNA) as their genome Their mRNA is produced by transcription in much the same way as with cellular DNA, using the enzymes of the host cell Group II viruses have singlestranded DNA (ssDNA) as their genomeHowever, their relationships with singlestranded DNA (ssDNA) viruses have not yet been defined in detail We report the isolation and
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Feb 26, 19 · A double stranded DNA virus enters the host nucleus before it begins to replicate It makes use of the host polymerases to replicate its genome, and isNov 17, 03 · Adenoassociated virus (AAV) is a promising gene vector based on a singlestranded (ss) DNA virus Its transgene expression requires the conversion of ssDNA to doublestranded (ds) genome, a slowSolution for The doublestranded circular DNA molecule thatforms the genome of the SV40 tumor virus can be denatured into singlestranded DNA molecules
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These discontinuities represent the priming sites for DNA synthesis8VF The forms of viral DNA are indicated as OC dsDNA open circular doublestranded (ds) DNA, SC dsDNA (supercoiled dsDNA), and circular singlestranded (ss) DNA A 379bp DNA fragment of Rep was PCR amplified and labeled with α32P dCTP and used as a probe Viral particles observed under transmission electron microscopyHuman papillomavirus (HPV) is a small, nonenveloped deoxyribonucleic acid (DNA) virus that infects skin or mucosal cells The circular, doublestranded viral genome is approximately 8kb in length The genome encodes for 6 early proteins responsible for virus replication and 2 late proteins, L1 and L2, which are the viral structural proteins
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Feb 04, · Of the novel circular sequences detected in the survey, 1844 encode genes with similarity to proteins of ssDNA viruses and 55 encode genes with similarity to dsDNA viral proteins ()The large majority of genomes from this study are highly divergent from RefSeq entries (Figure 1—figure supplement 1)We discovered 868 genomes that had similarity to unclassified eukaryotic virusesHBV is a hepatotropic DNA virus approximately 42 nm in diameter with particles consisting of surface proteins and core proteins 2 surrounding a partially doublestranded, relaxed, and circular 32 kb genomic DNA component (called rcDNA) 3 (additional details can be found in other reviews 4) During its natural life ,TT virus (TTV) is an unenveloped, circular, and singlestranded DNA virus commonly infecting human beings worldwide TTV DNAs in paired serum and liver tissues from three viremic individuals were separated by gel electrophoresis and characterized biophysically TTV DNAs in
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The supercoiled, circular, singlestranded DNA genome of about 45– kb, encodes 4–13 proteins Viruses of this family infect cell wallless bacteria, adsorbing to the bacterial surface, replicating their DNA by a rollingcircle mechanism or transposition, and releasing progeny from cells by extrusion, without killing the hostJun 18, 14 · A field guide to eukaryotic circular singlestranded DNA viruses insights gained from metagenomics Arch Virol 157, 1851–1871 (12) CAS PubMed Google ScholarThe family name is taken from the disease caused by members of the group ie, Papilloma, Polyoma and Vacuolating viruses These are small DNAcontaining viruses The DNA is doublestranded and circular and contains five to eight genes The virion is naked icosahedron with 72 capsomers Members of this family include
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